MODULATION OF INFLAMMATORY MEDIATORS INVOLVED IN EXPERIMENTALLY INDUCED HEART FAILURE BY USING OMEGA-3 FATTY ACIDS
Asaad N. Jassimaa, Ahmed A. Shaabana, Awny H. Yaseenb, and Tarek M. Ibrahima
aDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura
bDepartment of Histology and Cytology, Faculty of Medicine, Mansoura University, Mansoura
Heart failure is a complex progressive clinical syndrome. It results from many structural changes in the ventricles or functional disturbances, which reduce the heart ability to fill or eject blood from its chambers because of the defect in the normal architecture of the left ventricle of the heart. Four groups of adult male wistar rats weighing (200 - 250 gm) were used in this study. Group I (control): rats were allowed free access to water and standard laboratory food for 4 weeks without any drug, group II doxorubicin (Doxo): received Doxo HCl injection (2.5 mg/kg) every 2 days for 2 weeks to induce heart failure, group III carvedilol + doxorubicin (Carv + Doxo): contain three sub groups, injected with Doxo HCl for 2 weeks to induce heart failure, then take Carv orally for another 2 weeks in the following daily doses (30 mg/kg, 15 mg/kg and 7.5 mg/kg), with free access to water and standard laboratory food, group IV (omega+Doxo) injected with Doxo HCl for 2 weeks to induce heart failure and take daily omega-3 fatty acids (150 mg/kg) orally started with Doxo injection, after that, rats continue receiving only omega-3 fatty acids (150 mg/kg/day) orally for another 2 weeks, with free access to water and standard laboratory food. Biochemical evaluation was done. Omega-3 fatty acids resulted in a remarkable decrease in the mortality rate caused by Doxo injection. Doxo induced cardiac injury indicated by a significant increase in serum creatine kinase-MB (CK-MB) activity, serum lactate dehydrogenase (LDH) activity and serum alkaline phosphatase level (ALP). Omega-3 fatty acids administration decreased these markers indicating its beneficial effect.
December 2016