ACRYLAMIDE INDUCED LIVER TOXICITY AND THE POSSIBLE PROTECTIVE ROLE OF VITAMIN E IN ADULT MALE ALBINO RAT: HISTOLOGICAL, BIOCHEMICAL AND HISTOCHEMICAL STUDY
Soheir H. El-Sharouny, *Ayman A. El Enein Rizk, Sherif F. Arsanios Tamer M. Shawky, Ahmed H. Bayoumy
Department of Anatomy and Embryology Faculty of Medicine Cairo University
Background: The liver is a commonly affected organ by acrylamide toxicity which might be due to oxidative stress. Vitamin E is the most important lipid-soluble antioxidant as it protects cell membrane from oxidation by reacting with lipid radicals produced in the lipid peroxidation chain reaction. Aim of work: was to investigate the hepatotoxic effect of acrylamide and evaluate the possible protective role of vitamin E in adult male albino rats. Material & methods: 40 adult male albino rats were used in this study. They were randomly divided into 4 equal groups: group I (control group), Group II (sham control), Group III (acrylamide-treated group) and Group IV (acrylamide and vitamin E treated group). At the end of experimental period, blood samples were obtained for measuring the liver enzymes, then the rats were sacrificed by cervical dislocation and the liver of each animal was extracted and prepared for light microscopic, biochemical and histochemical study. Results: histological sections of group III revealed marked pathological changes in the liver in the form of dilatation and congestion of the central vein and hepatic sinusoids. The hepatocytes showed nuclear degeneration and rarefaction of their cytoplasm. Liver sections revealed weak PAS reaction. There was marked increase in the liver enzymes and the oxidative markers in the liver. Treatment with vitamin E in group IV resulted in improvement in the structure and function of the liver. Conclusion: vitamin E could protect the liver from acrylamide induced toxicity in adult male albino rat.
December 2016