EJMS

Abstract

Introduction: The most commonly prescribed class of antibiotics is the aminoglycoside family. Gentamicin is the most often used member in clinical practice. Although serum level is in the therapeutic range, aminoglycoside nephrotoxicity might still happen, it is perhaps the most prominent adverse effect associated with aminoglycoside therapy. Saffron has been shown to be an effective anti-oxidant and anti-inflammatory in the fight against oxidative stress. Aim of study: the current research may highlight saffron protective role against nephrotoxicity brought by gentamicin in rats. Materials and methods: Four equal groups of forty male albino rats were established. Group I (Control group) received 0.3 ml distilled water I.P. daily .Group II (the saffron group) has got 80 mg/kg I.P. daily of aqueous saffron extract. Group III (Gentamicin group) received 70 mg/kg/day I.M. Gentamicin and aqueous saffron extract were administered to Group IV (Gentamicin plus saffron group). The course of treatment lasted 14 days. For All groups, renal cortex specimens underwent light microscopic processing. After conducting quantitative morphometric analyses, the results were statistically analyzed. Samples from all groups have taken and checked for urea and creatinine. Results: Rats given gentamicin revealed an increase in blood urea and creatinine as well as acute tubular necrosis, whereas rats given saffron exhibited a decrease in serum urea and creatinine levels as well as a lesser degree of necrosis. Saffron-treated rats did not differ from control rats in any biochemical indicators. Rats receiving gentamicin had severely congested kidneys that also had vacuolation, desquamation, and interstitial mononuclear cell infiltration. Collagen fibers were strongly deposited, as seen by Masson's trichrome staining. Caspase-3 immunoreactivity was highly positive. Saffron ameliorates these alterations. Conclusion: In the rat renal cortex, aqueous saffron extract cause attenuation to histological alterations-induced by gentamicin.