TOLL LIKE RECEPTOR 2 (-196 TO -174 DELETION) POLYMORPHISM COULD INCREASE THE RISK OF URINARY BLADDER CANCER IN PATIENTS WITH SCHISTOSOMA HAEMATOBIUM INFECTION
Samar A. El-Refai1, Mohamed A. Helwa2, Shereen M. Fathy3, Selim M.4
1Parasitology Department, Faculty of Medicine, Menoufia University.
2Clinical Pathology Department, Faculty of Medicine, Menoufia University.
3Pathology Department, Faculty of Medicine, Menoufia University.
4Urology Department, Faculty of Medicine, Menoufia University.
Background: Bladder cancer (BC) is one of the most common cancers diagnosed in the world. Bladder schistosomiasis is an important risk factor for bladder cancer. Toll like receptor 2 (TLR2) polymorphisms may increase the risk of such cancer especially in Schistosoma (S.) haematobium infected patients. Objectives: This study was done to investigate the role of TLR2 -196 to -174 deletion (del) polymorphism in development of BC in S. haematobium infected patients. Subjects and methods: This case control study included 94 subjects divided into 3 groups: group I (44 cases of BC on top of S. haematobium infection), group II (20 cases of BC without evidence of schistosomiasis) and group III (30 healthy controls). Urine samples were taken from all participants in all groups and examined by centrifugation method for egg detection and by urine-circulating cathodic antigen (CCA) test for detection of schistosomal antigen. Blood samples were drawn from all participants in all groups and collected in EDTA tubes and kept at -20°C until used for studying of TLR2 polymorphism (-196 to -174 del) by a polymerase chain reaction (PCR). Results: Bladder cancer patients with S. haematobium were younger than those without schistosomiasis. The male/female ratio of BC patients was 3:1. Patients with urinary schistosomiasis and TLR2 (-196 to -174 del) del/del genotype had a statistically higher risk of development of BC when compared to those without schistosomiasis and to the healthy control subjects. Conclusion: TLR2 (-196 to -174 deletion) polymorphism; del/del genotype increases the risk of BC in patients with urinary schistosomiasis.