COMPARATIVE STUDIES AND EVALUATION OF ANTIPARASITIC DRUGS (TINIDAZOLE) ON IMMUNE RESPONSE OF HUMAN AND ANIMALS INFECTED WITH INTESTINAL PARASITES
Alaa Abd El-Aziz Mohammad Samn
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
This study included a total of forty male rats divided into six groups. The first groups as control. The groups from second into six were divided into 8, 15, 22, 29 and 35 post-infection groups. Serum levels of TNF-α, IL-4, IL-10 and IgE were determined. Also eosinophil and trophozoites count were determined to evaluate the immune responses in human and rats infected with E. histolytica after treatment with tinidazole. Immunological evaluation was performed by measurement of secreted cytokines (interleukin, IL-10-tumor necrosis factor-α (TNF-alpha) in blood of man, on the other hand E. histolytica cyst output in person stool was measured. The present investigation is directed to study the therapeutic effect of different doses of tinidazole, a treatment known for controlling of E. histolytica infection in infected persons. The infected person were divided into three group according to the dose of tinidazole (Fasigen syrup). The first, second and third group were treated with 30, 25 and 15 mg /kg per day for 7 days, in 3 doses respectively. The results of the present study revealed a significant reduction in cyst count in stool of person treated by tinidazole at dose of 30 mg /kg in comparison with control untreated. The data also revealed that serum IL-10 and TNF-alpha were significantly increased as an immunological response for infected person treated with tinidazole at dose of 30 mg/kg body weight in comparison with person infected treated with dose of 15 mg and untreated control group. Also the NO level revealed significant decrease in infected person at dose 30 mg / kg in comparison with 25 and 15 mg/kg group. TNF-α, IL-4 and IL-10 levels were significantly increased in all infected groups as compared with control. On the other hand, eosinophil count recorded a high level percent change in all infected groups as compared with control. Also total IgE was highly significantly increased in all infected groups (8, 15, 22, 29 and 36 day post Infection) with percent change 137.7 %, 175.6 %, 208.7%, 247.5% and 263.3% respectively as compared with control.