EJMS

Abstract

Background: Estrogen is a major regulator of wound repair that can reverse age related impaired wound healing in human and animal models. Tamoxifan (TAM) acts as an estrogen agonist in uterus and an estrogen antagonist in breast. It has beneficial effect on wound healing. Despite clearly different functions in some tissue (e.g. uterus), TAM acts as estrogen agonists (i.e. potentially accelerates cutaneous wound healing) in the skin. Cell migration is a key element of wound healing process and a recent study has shown that tamoxifen induces cytoskeleton remodeling and migration. Aim of the work:The present work was designed to study, compare and contrast the effect of tamoxifan on wound healing of Senile, Adult Ovariectomized and Adult Non-ovariectomized Female Rats. Material and Methods:The study will be carried out on thirty adult (8 month old) non pregnant female albino rats, twenty senile (16-18 month old) female albino rats and twenty adult (8 month old) male albino rats.Twenty of the adult female rats will be ovariectomized through abdominal incision one month before the start of the experiment.The animals will be divided into six main groups; Group I ( control group), Group II (control senile group), Group III( ten ovariectomized young adult female rats) ,Group IV (ten ovariectomized young adult female rats receiving Tamoxifen, Group V( ten non-ovariectomized young adult female albino rats receiving Tamoxifen, Group VI (ten non-ovariectomized senile female rats receiving Tamoxifen.Tamoxifen was administered orally at doses of 0.5 mg/kg daily for seven days before skin wounding and five days after skin wounding in Groups IV,V,VI. Wounds were made bilaterally. After fixation and routine processing, histological sections will be prepared from the wounded tissue. Sections will be prepared for histological and histomorphometric study. Results: Light microscopic examination of the the wounded skin of group II and III showed the epidermis with incomplete re-epithelialization. A large wound gap was left with a scab which was still present. The thin keratin layer migrated beneath the scab that never bridged the whole incision. The dermis appeared with large area of granulation tissue which had numerous inflammatory cells. Disorganized collagen fibers appeared in the dermis as large thick randomly distributed fibrils. Examination of wounded skin of group IV ,V ,VI displayed improvement of the histological changes. Conclusion: Tamoxifen has a beneficial role in wound-healing that makes it recommended as a concomitant factor during wound treatment.