EJMS

Abstract

Background: Treatment with the anti-arrhythmic drug Amiodarone (AD) is frequently associated with thyroid dysfunction. Green tea extract supplementation might attenuate oxidative stress and thus would ameliorate AD-induced thyroid cytotoxicity. Aim of work: The present study aimed at investigating the possible protective effect of green tea (GT) on amiodarone-induced thyroid damage in albino rats. Material and Methods: Twenty four adult male albino rats were divided into 4 groups: Group 1 (Control Group) n= 6 rats. Group 2 (Green Tea Group) n=6 rats, each rat was given 50 mg/kg of GT extract orally. Group 3 (AD Group) n=6 rats, each rat was given AD 30 mg/kg daily orally. Group 4 (AD and GT Group) n= 6 rats, each rat was given AD 30mg/kg and GT extract 50mg/kg daily orally. Thyroid function tests were performed. Rats were sacrificed after 6 weeks. Thyroid sections were subjected to histological, histochemical, immunohistochemical and morphometric studies and results were statistically analyzed. Results: There was a significant increase of T3 and a significant decrease of TSH in AD group compared to other groups. Thyroid sections of green tea-treated rats (group 2) showed histological results comparable to the control group. Group 3 showed multiple follicles with little colloid with large vacuolations while some follicles were devoid of colloid. In group 4, some follicles presented little colloid with large vacuolations. There were a significant increase in the area % of collagen and PCNA and a significant decrease in colloid area % in AD group compared to other groups. Conclusion: Green tea has an ameliorating effect on the amiodarone-induced thyroid damage.