EVALUATION OF FOUR DNA EXTRACTION METHODS SEEKING FOR SUCCESSFUL AMPLIFICATION OF S. HAEMATOBIUM GENOMIC MATERIALS IN URINE SAMPLES FROM INFECTED CASES
Mohamed S Badr1, Hala M. El-Askary2*, Marwa Ahmed El-Dardiry3, Dina M. Hamdy El Akkad4, AbdAllah M.A. El Ebidi5, Hesham M. Hefny6, Ahmed N.M. Salem6, And Samia El-Sahn7
1Department of Microbiology and Molecular Biology, Faculty of Medicine, Ain Shams University.
2Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University.
3Department of Medical Parasitology, Faculty of Medicine, Fayoum University.
4Department of Medical Parasitology, Faculty of Medicine, Cairo University.
5Department of Medical Biochemistry, Faculty of Medicine, Aswan University.
6Department of Clinical Pathology, Faculty of Medicine, Sohag University.
7 Department of Pediatric, Faculty of Medicine, Ain Shams University.
Schistosomiasis demands a reliable diagnostic tool which is sensitive enough to detect low worm burden. Molecular techniques based on polymerase chain reaction (PCR) are now recommended as an alternative for diagnostic purposes. Reliable unbiased extraction of nucleic acid is a key issue in the sensitivity and usefulness of biological analyses for diagnosis of parasitic diseases. This study compared the relative efficacy of extraction of Schistosoma nucleic acid from urine using four simple and feasible DNA extraction techniques. The measurements of DNA yield and purity exhibited variations between the four methods tested in this study. Our results revealed that magnetic beads method displayed the best quality and highest quantity of DNA compared to the other methods assessed with the most efficient amplification reaction which allows the diagnosis of S. haematobium disease in easily obtainable urine samples.
December 2013