THE EFFECTS OF A CYTOLYTIC PROTEIN VIB ISOLATED FROM NAJANIGRICOLLIS NIGRICOLLIS VENOM ON MICE TISSUES
Mahmoud Tag1 Dalia Gaber2, Ayman Basheer, 3, Kamelia Zaki2 and M. Farid El-Asmer3
Departments of Pathology1 and Biochemistry2, Faculty of Medicine, Misr University for Science & Technology Department of Biochemistry, Faculty of Medicine, Ain Shams University3
Objective: to evaluate the effects of cytolytic protein VIb on mice hepatic, renal and bone marrow tissues to assess the use of this cytolytic protein as adjuvant biotherapeutic agent for cancer treatment. Material and methods: purified cytolytic protein VIb was injected intraperitoneally in mice. The liver, kidney and bone marrow of such animals were dissected and examined for any histological changes. Also EM study of liver tissue was done in some cases. Results: in a dose of 20 and 40µg, VIb protein caused degenerative changes in both liver and kidney tissues. These changes were more pronounced in higher dose. 40µg of same protein caused hypercellular marrow. EM study was done in liver tissue treated with 40µg and revealed mitochondrial changes. Conclusion: protein VIb is a cytolytic protein, isolated from crude Naja nigricollis nigricollis venom. In relatively large doses this protein causes degenerative changes in liver and kidney tissues. In addition it causes hypercellular bone marrow.
December 2011