GENOTYPING OF GIARDIA DUODENALIS IN CHILDREN BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM Nadia Salah Elnahas
Parasitology Department, Faculty of Medicine, Menoufiya University
Genetic characterization of G. duodenalis isolates has revealed the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are found in humans and in many other mammals. The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of G. duodenalis. To determine the distribution of genotypes of G. duodenalis in children in Menoufiya Governorate , 220 stool samples were collected from children attending rural health care units in different localities of Menoufiya Governorate, and examined by wet mount, formalin diethyl ether, acridin orange and modified ziehl neelsen stains, 28 positive specimens were analysed using PCR and restriction fragment length polymorphism (RFLP). The results showed that assemblage B was more prevalent, it was detected among 17/28 (60.7%) children, while assemblage A detected in 11/28(39.2%) ones.
December 2011