PHYTOCHEMICAL AND PHARMACOLOGICAL STUDIES OF CROTALARIA MADURENSIS LEAVES
Magada TI1, Mohamed SAM2, Seham AE3, Hala SHM1, Omayma DE1
1Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
2Natural Product Research Group, National Research Centre, Cairo, Egypt.
3Pharmacology Department, National Research Centre, Cairo, Egypt.
Chromatographic separation of aqueous MeOH extract of the leaves of Crotalaria madurensis Wight & Arn has yielded two triterpenoidal saponins have been identified as 16-β-hydroxy-β-amyrin 3-O-β-glucopyranosyl- (1→6)-O-β-glucopyranosyl- (1→4)-O-β-glucuronopyranoside (1) and 16-β-hydroxy-β-amyrin 3-O- [4'-O-β-glucopyranosyl- (2'-β-glucopyranosyl) glucuronopyranoside] (2), together with three flavonoidal glycosides, i.e. isorhamnetin 3-O-β-D-4C1-galactopyranoside (3), kaempferol 3-O-β-D-4C1-galactopyranoside (4) and Quercetin 3-O-β-D-4C1 galactopyranoside (hyperin, 5). Their structures were estadlished on the basis of chromatographic properties and spectroscopic analysis (UV, 1H and 13C NMR). The investigated aqueous methanol (AME) and chloroform extracts (CE) were examined as anti-inflammatory, analgesic, gastric ulcerogenic and antioxidant activities.
December 2011