EJMS

Abstract

Early diagnosis of Trichomonas is very critical for inhibition of transmission of the disease. Trichomoniasis is usually associated with urinary tract infection and empirical use of antibiotics cause antibiotic resistance. This study investigated the associated co- infection with Trichomonas in the genital system, urinary tract and the best sample for screening of the infection. This study was carried out on 802 women selected from patients attending Outpatient Clinics of the Obstetric & Gynecology in female hospital in Sakaka ranging in age from 20- 65 years complained of profuse vaginal discharge associated with urinary symptoms (dysuria, frequency) and100 female selected apparently healthy (controls). Two samples were taken from each anatomical site (vagina, cervix) one sample for wet mount and another for swab in a swab tube. One urine sample in sterile container is also taken from each female. Culture for vaginal and cervical swab on blood agar, macConkey, chocolate and sabaureau media. Urine cultures were done if pus cells more than 10 /HPF, on blood agar, CLED and macConkey media. Identification of organism and sensitivity to drugs would be done by microscan walkaway 96 plus. Candida is the most prominent associated infection with Trichomonas, the detection was 15.7% while Trichomonas 13%. Vaginal samples were superior to all other samples type in detection of the infection with Trichomonas and the sensitivity of cervical and urine samples compared to vaginal samples were 68.5% and 52.3% respectively. Out of 105 cases were affected by trichomoniasis, there were 87 cases associated with pyuria but after urine culture 75 % had sterile pyuria. In conclusion, while testing for Trichomonas, vaginal samples were the sample of choice. There were overlap between Trichomonas and Candida in clinical presentation. There were also overlap with urinary tract infection but there were no need for empirical use of antibiotics as most cases had sterile pyuria.