ELECTROPHORETIC AND HISTOLOGICAL STUDIES OF HAMSTER (MESOCRICETUS AURATUS) INFECTED WITH SCHISTOSOMA HAEMATOBIUM CERCARIAE
Fayez A.Bakry1, Hoda M. Shoman2, Nadia F. Ebrahim2, Ghada A. Saad3, Entsar R. Farag2
1Medical Malacology,Theodor Bilharz Research Institute (TBRI), Giza, Egypt, 2Zoology department, Faculty of Science (for girls), Al-Azhar University, Cairo, Egypt 3Parasitology department, Faculty of Medicine, Ain Shams University
The present work evaluated the effect of infection with Schistosoma haematobium cercariae on parasitological and biochemical parameters as well as histological structure on liver of hamster. The present results showed viable bilharzial egg deposition, with related granulomas of chronic inflammatory cellular collection having a portal location in the liver of hamster infected with S. haematobium cercariae. Electrophoretic analysis of liver homogenate’s total protein showed a remarkable deviation (40.44%) in the liver homogenate of hamsters infected with S. haematobium in comparison with the control non-infected group. In addition, there was a remarkable deviation (39.32%) in the serum homogenate of hamsters infected with S. haematobium in comparison with control non-infected group. Also, the activity of transaminase enzymes (Aspartateami-notransferase (AST) and Alanineaminotransferase) in serum and liver tissue of infected hamsters showed a significant decrease than that in the control hamsters. In conclusion, S. haematobium causes serious histopathological changes on liver of hamsters as well as protein profile both of liver homogenate and serum.