THE INFLUENCE OF PLATELET-RICH PLASMA (PRP) IN AMELIORATING CISPLATIN INDUCED TESTICULAR DAMAGE. A HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY
Shereen Abdel Fattah1 , Tarek Ibrahim Abd El-Galil1
1Department of Anatomy and Embryology, Faculty of Medicine, Cairo University
Background: Cisplatin is an extensively utilized anticancer drug. Several studies propose that it induces testicular impairment. This study was conducted to evaluate the modulatory influence of Platelet-rich plasma (PRP) on cisplatin induced testicular harm, as PRP was not used before in e protection of testes against cisplatin grievance Materials and methods: Thirty rats were divided into four groups: control, sham control cisplatin- administered, cisplatin-PRP administered, the residual six rats were intended for the preparation of PRP. Light microscopic (H&E and masson trichrome), immunohistochemistry (iNOS and TNFα), oxidative/antioxidative stress markers (MDA/GSH and SOD), real time polymerase chain reaction (SOD2 and NF-Kb), morphometric and statistical analyses were done. Results: cisplatin treated group revealed shrunken, degenerated seminiferous tubules, detachment and vacuolations of the germinal epithelium. Blood vessels were congested. Disrupted leydig cells, gapping, and fibrosis between semineferous tubules were also perceived in the same group. Also, the cisplatin group showed a significant increment in immune-expressions of iNOS , TNF-α, mean values of MDA and Nf-kb, but revealed significant decline of SOD, GSH and SOD2 values when matched to the control. PRP had almost restored the testicular architecture and exhibited a significant decrement in the immune-expressions of iNOS and TNF-α, and mean values of MDA and Nf-kb, but exposed significant surge of SOD, GSH and SOD2 values if related to the cisplatin group Conclusions: The deleterious belongings of cisplatin on testis might be accredited to either oxidative process or severe inflammation. PRP is competent in improving these harmful possessions
December 2018